Cryopreservation of Boar Spermatozoa: An Important Role of Antioxidants
نویسنده
چکیده
Artificial insemination (AI) is one of the first reproductive biotechnologies has been established and developed in the pig production system. In most case, liquid stored semen or fresh semen is used for AI in commercial swine herds (Wagner and Thibier, 2000). The use of FT boar semen for AI is limited due to the low fertility outcomes compared to extended fresh semen (Johnson et al., 2000; Wagner and Thibier, 2000). The first success of boar semen cryopreservation was reported in 1956 (Polge, 1956) and the first pregnancy was achieved with FT boar semen using surgical insemination in 1970 (Polge, 1970). Currently, the attempt to develop the boar semen cryopreservation technique is ongoing. Nevertheless, the success of boar semen cryopreservation is relatively variable because the factors responsible for the cryosurvival of boar spermatozoa have not been entirely elucidated.
منابع مشابه
P-65: Low-Density Lipoproteins Extractions on Boar Spermatozoa Quality Following Freezing–Thawing
Background Low-density lipoproteins (LDL) is known to protect boar sperm during freezing–thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing–thawing. Ma...
متن کاملEffects of hypothermic liquid storage and cryopreservation on basal and induced plasma membrane phospholipid disorder and acrosome exocytosis in boar spermatozoa.
Flow cytometry was utilised to determine whether short-term (Day 1) or long-term hypothermic liquid storage (Day 5), or cryopreservation of boar spermatozoa (1) caused changes in plasma membrane phospholipid disorder (MPLD) and acrosome exocytosis (AE), indicative of an advanced stage of capacitation or acrosome status, and (2) facilitated or inhibited the induction of capacitation and the acro...
متن کاملAdvances in Boar Semen Cryopreservation
The present paper highlights aspects of the cryopreservation of boar semen, a species with particular large, fractionated ejaculates, and a cumbersome cryotechnology that had prevented its commercial application. With the dramatic increase of use of liquid pig semen for artificial breeding over the past decade, developments on cryopreservation alongside the routine use of stud boar semen for AI...
متن کاملP-73: Effect of Sodium Dodecyl Sulfate and Different Levels of Clove Bud Extract on Motility, Viability and Plasma Membrane Integrity of Ram Spermatozoa during Cooling and Cryopreservation
Background: Hen egg yolk is a common component of most semen cryopreservation extenders. However, diluents containing the egg yolk may have adverse effects on the quality of the semen. Egg yolk could be solubilized by the addition of sodium dodecyl sulphate (SDS) in semen extenders. Many studies have shown beneficial effects of antioxidants addition to semen freezing extenders. Antioxidants are...
متن کاملInteractions of egg yolk lipoprotein fraction with boar spermatozoa assessed with a fluorescent membrane probe.
The interactions of a fluorescent membrane probe, 1-anilinonaphthalene-8-sulfonic acid (1,8-ANS), with boar spermatozoa were followed through the use of lipoprotein fraction of ostrich egg yolk (LPFo). Semen samples, extended in Kortowo 3 (K3) extender, were supplemented with 2% or 5% LPFo and stored for 3h at 16 degrees C. Additionally, cold shock-treated spermatozoa (1h at 4 degrees C) were s...
متن کامل